The invention relates to an integrated safety measure test junction box and use method. The integrated safety measure test junction box includes a connector socket, working plugs and test plugs, current sampling metal pieces, current short circuit metal pieces and a grounding metal piece are arranged in a socket shell cavity of the connector socket, the current sampling metal pieces are connected with a current transformer secondary side and a current line of a relay protection device, the grounding metal piece is connected with a grounding cable of the relay protection device, and current sampling jacks and loop normal work jacks are arranged in the socket shell; the working plugs are inserted in the loop normal work jacks to be connected with the current sampling metal pieces, and the working plugs are pulled out of a secondary side loop of the current transformer and are shorted with the current short circuit metal pieces; and one ends of the test plugs are connected with an on-site calibration instrument, and the other ends are inserted into the current sampling jacks. The integrated safety measure test junction box provided by the invention realizing automatic safety measures and isolation of a secondary loop by use of pulling-out and insertion of the working plugs, and the on-site calibration instrument is connected to the relay protection device through the test plugs, thereby completely eradicating potential safety hazards and safety accidents caused by manual misoperation.