Disclosed are a display substrate and a manufacturing method therefor, and a display panel comprising the display substrate. The display substrate comprises base substrates (51, 61, 71, 81) and an optical film layer (3) formed on the base substrates (51, 61, 71, 81). The optical film layer (3) is configured to filter light having a wavelength within a selected wavelength range. The display substrate may be an array substrate (1) or a colour film substrate (2).