The invention relates to a rodent pest prevention method of a traditional Chinese medicinal material land, migration and diffusion ways of subterranean rodents are interdicted by using a steel wire gauze to divide a traditional Chinese medicinal material cultivated land into regions, detection on shockwave by the subterranean rodents is interfered by the steel wire gauze , marriage and breeding and normal activities of the subterranean rodents are restricted. The biology characteristics and ecology characteristics of a zokor are combined by the invention, the living space is narrowed down by using the steel wire gauze divided regions to interdict the migration way; the detection on the shockwave by the zokor is interfered by the steel wire gauze, so that normal marriage and breeding and the like of the zokor are effectively prevented, the growth speed of the population is decreased; the rodent pest prevention and control effect is improved by the steel wire gauze divided regions control, and migration and invasion of exogenous rodents resource such as surrounding farmland and the like are also prevented. By means of performing fixed-point artificial elimination and fixed-point medical prevention under the steel wire gauze divided regions space, the defects of the existing prevention method is overcome so that the aim of effective prevention on the traditional Chinese medicinal material cultivated land subterranean rodent pest is achieved. The invention has the advantages that traditional Chinese medicinal materials loss and harm caused by the subterranean rodents are reduced, and the yield of the traditional Chinese medicinal materials is greatly improved.