The invention discloses exclusive bacteria and a technology for removing organic sulfur in high sulfur coal mine through the exclusive bacteria. The exclusive bacteria have a class name of Bacillus subtilis Retech Organosulfur-I, are preserved in the China general microbiological culture collection center in the microbiology institute with the address of No. 3, Beichen West Road, Chaoyang District, Beijing, China on the collection date of May 28, 2015, and has a preservation number of CGMCC No. 10933. The bacillus can degrade the organic sulfur in the coal mine under acidic and aerobic conditions, and through combination of the bacillus and the high efficiency desulfurization mixed strain Retech TS-I, inorganic sulfur is removed and effective total sulfur removal of high sulfur coal mine is realized. Compared with the conventional physical and chemical methods, the technology has the advantages of mild conditions, low cost and no harmful gas emission, and has a wide application prospect in the field of coal mine desulfurization.