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发明授权
CN106544339B 一种用于提取动物组织DNA的试剂盒 有权

一种用于提取动物组织DNA的试剂盒
摘要：
一种用于提取动物组织DNA的试剂盒，该试剂盒包括有提取液，提取液中含有磁性微球、异硫氰酸酯、盐酸胍、碘化钠、EDTA、十二烷基氨基酸，其中异硫氰酸酯的浓度为3.5‑5M,盐酸胍的浓度为1.5‑3M,碘化钠的浓度为1.2‑2.5M,EDTA的浓度为15‑30mM,十二烷基氨基酸的浓度为60‑100mM；磁性微球的质量浓度为10‑15％。根据本发明的用于提取动物组织DNA的试剂盒，能够对动物组织进行快速解离并高效分离提纯DNA，能够广泛应用到动物疫病检测领域。
摘要（英）：

Disclosed is a kit used for extracting animal tissue DNA. The kit comprises an extracting solution. The exacting solution comprises magnetic microspheres, isothiocyanate, guanidine hydrochloride, sodium iodide, ETDA and dodecyl amino acid, wherein the concentration of the isothiocyanate is 3.5-5 M, the concentration of the guanidine hydrochloride is 1.5-3 M, the concentration of the sodium iodide is 1.2-2.5 M, the concentration of the EDTA is 15-30 mM, the concentration of the dodecyl amino acid is 60-100 mM, and the mass concentration of the magnetic microspheres is 10-15%. According to the kit used for extracting animal tissue DNA, rapid dissociating of animal tissue and efficient separating and purifying of the DNA can be achieved, and the kit can be widely applied to the field of animal epidemic disease detection.

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