The invention discloses a rooting method of eucalyptol type oil camphor tree tissue cultured buds. The method comprises the following steps: selecting eucalyptol type oil camphor tree subcultured buds obtained after 30-35 d bud strengthening culture in tissue culture, shearing strong single buds with the height being not less than 2 cm from subcultured bud clusteres, inserting the single buds into an aseptic solution of L-cysteine, immersing the single buds to preprocess the single buds, vertically inserting the preprocessed single buds into a rooting medium, carrying out rooting culture in specific light and temperature environment, moving the rooted buds to a seedling growing shed with natural environment conditions, and hardening seedlings to obtain eucalyptol type oil camphor tree seedlings. The method solves the problems of difficult rooting, low rooting rate, long rooting time, small root number and easy blackening of roots of the eucalyptus oil-type camphor tree subcultured buds, and has the advantages of short rooting period, high rooting rate, large root quantity, good quality, high transplanting survival rate of tissue-cultured and rooted seedlings, realization of tissue culture industrial seedling growth, provision of high quality seedlings for construction of a natural perfume artificial clonal forest, and good economic, social and ecological benefits.