The present invention discloses an ACF press welding temperature detection method. A thermometer is arranged on a thermocouple which is disposed on ACF glue to make a clamp for testing the temperature, the clamp is putted under a circuit board, a heating head is arranged at the upper portion of the circuit board, a glass board is arranged at the lower portion of the circuit board, the glass board is configured to arrange the circuit board, a rubber thin film is arranged between the heating head and the circuit board, the heating head is configured to heat the circuit board to simulate the press welding in the production process, the thermometer is configured to detect and obtain the conduction temperature of the ACF glue, and the real conduction temperature of the DCF is detected and obtained through completely simulating the press welding in the ACF production process. The structure is simple, the operation is convenient, the detection precision is high, and the detection cost is low.