The invention discloses a method for detecting influence on cell catalase enzyme vitality by an electronic cigarette product. The method comprises the following steps: pre-treatment on a test substance, preparation of a human lung fibroblast single-cell suspension, calculation of human lung fibroblast cell concentration, inoculation of human lung fibroblast cell, grouping of the test substance, incubation of the test substance, collection of a test substance incubation product, cracking centrifugation of the test substance incubation product, determination of a standard curve, determination of the test substance incubation product, and protein concentration determination and calculation of the catalase enzyme vitality. According to the effective processing on a sample, optimization setting of detection dosage and correct selection of the target cells, so that the method can accurately and effectively detect the influence on the cell catalase enzyme vitality by the electronic cigarette product.