The invention discloses a freezing-separation method of a gum base component in gum-base tobacco, comprising the following steps: (1) extruding and mixing the gum-base tobacco in a chewing simulator containing artificial saliva; (2) collecting the liquid component obtained after the extruding and mixing in the step (1), and carrying out centrifugal separation; (3) adding NaCl, alkali liquor NaOH and a low-polarity organic solvent into the centrifugate obtained in the step (2) and uniformly dispersing, standing, and collecting an organic phase; (4) freezing the organic phase obtained in the step (3) at low temperature; and (5) collecting the organic phase obtained in the step (4) for detection. The separation method of the invention is simple and fast to operate, and has good repeatability.