The invention provides a preparation method and application of a carbonized rice hull powder particle control and flooding agent. The preparation method comprises the following steps: (1) weighing a certain amount of rice hull and putting the rice hull into a carbonization tank; (2) putting the carbonization tank into a constant-temperature curing box, adjusting heating temperature to 200-270 DEG C, and carrying out carbonization treatment on the rice hull at the constant temperature for 2-3 h; and (3) taking out the carbonized rice hull, cooling the rice hull and finally crushing and screening so as to obtain the carbonized rice hull powder particle control and flooding agent. According to the preparation method of the carbonized rice hull powder particle control and flooding agent, rice hull is commercially available in quantity, and the carbonization processing is simple and easy to operate. The preparation method belongs to refuse reclamation and is low-cost. In comparison with other control and flooding agents, the prepared carbonized rice hull powder particle control and flooding agent is nontoxic and harmless and is green and environment-friendly. After the carbonized rice hull powder particle control and flooding agent is blended with an aqueous solution of partially hydrolyzed polyacrylamide polymers according to a certain mass ratio, the profile control plugging rate of deep-bed formation large-pores can reach 90% and above.