The invention discloses a method for measuring pipotiazine palmitate related substances. According to the method, a chromatographic column filled with cyano-bonded silica gel taken as the filling agent is adopted; a UV detector is adopted, and a mixed solution of a potassium dihydrogen phosphate solution and acetonitrile is taken as the mobile phase to carry out gradient elution. The provided method can effectively separate pipotiazine palmitate from all known impurities (residual intermediates 1-5), eliminates the interference of various impurities generated during the synthesis and processing process on the product purity, and is capable of effectively and comprehensively controlling the quality of raw materials and preparations of pipotiazine palmitate. The method has the advantages of simple operation, strong specificity, and high sensitivity, can well detect the raw materials of pipotiazine palmitate and related substances in preparations, and is capable of reliably and effectively controlling the quality during the development, production, and processing process.