The invention relates to a method for tissue culture and rapid propagation of caulis piperis kadsurae. The method comprises the following steps: explant selection, explant sterilization, propagation and culture, rejuvenation, rooting and seedling integrated culture and test-tube plantlet transplantation. The method provided by the invention has the benefits that caulis piperis kadsurae seedlings are propagated through a plant tissue culture and rapid propagation technology, cannot be affected by seasons, substrates, scions and other factors and are produced throughout the year; a large quantity of caulis piperis kadsurae seedlings for field cultivation can be propagated within a shorter period of time; the caulis piperis kadsurae propagation coefficients and the seedling quality are obviously improved, and the production needs are met.