The invention discloses an enterobactin preparation used for animals, and a preparation method thereof. The enterobactin preparation is prepared through mixing, by weight, 1-2 parts of freeze-dried Enterococcus faecalis powder, 1-2 parts of freeze-dried lactic acid bacillus powder, 1-2 parts of freeze-dried bifidobacterium powder, 1-2 parts of freeze-dried Bacillus subtilis powder, 1-2 parts of freeze-dried Saccharomyces boulardii powder, 0.1-1 part of vitamin B2, 0.1-1 part of biotin, 1-1.5 parts of pantothenic acid, 1-1.5 parts of folic acid, 5-15 parts of fructo-oligosaccharide, 10-25 parts of water-soluble starch and 30-50 parts of mainfan stones. Mutual compatibility of all the components makes the enterobactin preparation promote growth, enhance the immunity of the immune system, improve the ecologic balance of intestinal beneficial microbe florae, prevent diarrhea of young animals and prevent constipation and postpartum diseases of female animals.