The invention discloses a treatment solution for a graphene film substrate, a preparation method for the treatment solution, a treatment method for the graphene film substrate and a preparation method for a graphene film. The treatment solution for the graphene film substrate is a water or ethanol solution of dopamine hydrochloride and has the concentration of 0.01wt% to 1wt%. The dopamine hydrochloride can be effectively dissolved in alcohols, water or other solvents. The preparation method for the graphene film comprises the steps: soaking a target substrate with the solution for a period of time, and then, laminating the target substrate and graphene/substrate/graphene or a graphene face of a glue film/graphene in a pressurized or heated manner, wherein during the laminating of the target substrate and the graphene/substrate/graphene, the metal substrate is required to be etched so as to form a target substrate/polymer/graphene structure; and during the laminating of the target substrate and the graphene face of the glue film/graphene, the target substrate/polymer/graphene can be formed by only ripping away the glue film. Due to the polymer, the problems in sheet resistance rise and sheet resistance uniformity caused by a transfer problem can be solved while the adhesive power between the target substrate and the graphene can be improved greatly.