The invention discloses a cell autophagy monitoring probe, a preparation method therefor and use of the cell autophagy monitoring probe. The structure of the cell autophagy monitoring probe is represented by a formula shown in the description. Cellular co-localization experiments confirm that the cell autophagy monitoring probe can be specifically located to a lysosome; and shown by two-photon fluorescence microscopic imaging experiments, the cell autophagy monitoring probe disclosed by the invention has relatively good infiltration to MCF-7, Hela cells and the like, can be used for detecting changes of polarity of the lysosome and is suitable for being located to the lysosome in cells and detecting the change condition of the polarity of the lysosome. The cell autophagy monitoring probe disclosed by the invention can be used for monitoring an autophagy process of the cells through instantly detecting the change of internal polarity of the lysosome.