The invention relates to a method for preparing a corn bud juice strain culture medium, and belongs to the agricultural technical field, and the method comprises the following steps: 1, taking raw materials of 100 grams of corn, 30 grams of white sugar and 20 grams of agar; 2, soaking the corn in water for 10 to 12 hours, then taking the corn out, putting into an incubator for accelerating germination at 28 to 30 DEG C for 3-4 days until corn buds grow to 0.7cm-1.2cm for standby use; 3, cutting for separating to obtain the corn buds, mashing the corn buds, squeezing to obtain juice, filtering and clarifying the juice; 4, adding the agar and the white sugar into the corn bud juice, increasing the temperature to 80 to 100 DEG C, stirring to dissolve into a mixed solution; 5, taking ten test tubes, at 50 to 70 DEG C using a dropper to absorb the mixed liquid to respectively dropwise add into the ten test tubes; 6, putting the bundle of test tubes into a sterilization box for high-temperature sterilization; and 6, after the completion of the sterilization, taking the test tubes out, at 60 DEG C placing the bundle of test tubes with the angle between the bottom surface of the test tubes and the plane being thirty degrees, standing to cool to 15 DEG C to 25 DEG C, and then solidifying into a corn bud slant culture medium.