The invention discloses a method for calculating power generation cost by using quotation information of a power generation enterprise based on matchmaking trading, and belongs to the technical field of electricity market supervision. The method comprises the steps of acquiring power generation cost calculating base data, building a residual requirement curve for each time of bidding, constructing a derivative of a power generation enterprise profit function to quotation, constructing a power generation cost calculation optimized mathematical model, and acquiring the power generation cost of each unit in the power generation enterprise by using a linear programming solver. The invention provides the method for calculating the power generation cost by using the quotation information of the power generation enterprise applicable to matchmaking trading, so that supervision of electricity market regulators on the electricity market is reinforced, and stability of the electricity market and operation stability of the electric power system are improved.