The invention discloses a method for breeding an improved variety of hemp through a shoot tip, belonging to the technical field of hemp plantation. The method comprises the following steps: plant screening, pretreatment and sterilization of cut embryo buds, cutting of leaf primordial, cultivation of tissue culture seedlings, domestication of the seedlings, transplanting of the seedlings, etc. The method provided by the invention overcomes the problem of great difficulty in large-scale production of hemp caused by low seed germination rate, long germination acceleration time, inconsistent germination time, difficulty in collection of hemp seed resources, incapability of remaining all the excellent properties of male parents and female parents due to variation of filial generations and the like in traditional seed propagation of hemp.