The invention relates to the field of tissue culture of plants, aims to provide a culture medium for indirect regeneration of high-bush blueberry somatic embryos and a culture method. The method includes the steps: preparing four culture media; taking stems with leaf stalks of sterile test-tube plantlets of high-bush blueberries under sterile operation conditions; performing low-temperature and low-light shallow liquid culture for calluses, and performing low-temperature and dark differentiation multiplication culture for semi-solidification culture media embryonic calluses of blueberries; performing full-light and normal-temperature differentiation seedling culture for solid media of the blueberry embryos, and performing rooting seeding culture. According to the method, multiplication speed of non-embryonic calluses is reduced in the induction process of the calluses, and a browning risk caused by high temperature and long-term non-subculture is reduced. Four types of cytokinin are matched in low concentration, callus states of embryonic calluses are effectively maintained, multiplication of the embryonic calluses after successful induction is facilitated, the culture media and other media cannot be adhered on rooting seedlings, transplanting efficiency is high, and labor cost is remarkably reduced.