The invention belongs to the field of artificial induction to parthenogenesis of Chinese sturgeons and particularly relates to a method for inducing Chinese sturgeon ova to perform gynogenesis through genetically inactivated Chinese sturgeon homologous sperms. The method comprises the steps of genetic material inactivation of Chinese sturgeon sperms, Chinese sturgeon ovum collection, insemination operation of the Chinese sturgeon sperms, chromosome doubling processing of Chinese sturgeon fertilized ova, breeding of gynogenesis inducing ova and identification of gynogenesis fry. High-homozygous-degree Chinese sturgeon fry can be produced by utilizing the method, an experiment material is provided for research on whole genome sequencing of the Chinese sturgeons, a fry source is provided for production of single-gender Chinese sturgeon fry, information is provided for disclosure of Chinese sturgeon gender determining mechanism, and technical support is provided for further research on parthenogenetic reproduction production.