The invention provides an insect specimen making device. The insect specimen making device comprises a water bucket, wherein a flow guide groove is mounted in the upper part side of the water bucket; the width of a groove opening of the flow guide groove is gradually reduced and the flow guide groove is inclined; the front end of the flow guide groove is connected with the water bucket; a water recycling tank is arranged at the lower part of the tail end of the flow guide groove; the water recycling tank is connected with the upper part of the water bucket through a pipeline with a water pump; a plate type conveyor belt is mounted at the tail end of the flow guide groove; limiting plates are mounted at two sides of the plate type conveyor belt; a conveying roller group vertical to the plate type conveyor belt is arranged at the end part of the plate type conveyor belt; the conveying roller group is provided with an insect stabbing platform; a nail gun is arranged at the upper part of the conveying roller group; the nail gun can be used for launching mosquito nails. The insect specimen making device provided by the invention can be used for preparing insect specimens in batches and insects with the same type and size are produced. Poisoned and killed insects are softened through the insect specimen making device and are prevented from artificial contact in a softening process, so that the poisoning risk is reduced. A nail inserting step does not need manual operation so that dangers of stabbing nails to cause injuries are avoided. The insect specimen making device has the advantages of high efficiency and safety in an insect making process.