The invention provides a positioning catheter for atrial septal puncture. The end, stretching into human body tissue, of the positioning catheter serves as a catheter working end, the catheter working end is provided with magnetic sensors, signal transmission lines are connected to the magnetic sensors, the other ends of the signal transmission lines extend to the exterior of the human body tissue along the positioning catheter and are connected with a signal receiving device, and the positioning catheter is arranged in an electric field. Accordingly, the positioning catheter can provide accurate judging information for a doctor to judge whether the end face position of the current positioning catheter working end is a determined puncture position or not, and therefore the puncture success rate can be increased.