The invention belongs to the field of medical apparatus, and particularly relates to a bone induction differentiated metal bone trabecula knee joint prosthesis and a preparation method thereof under the precision control using incremental manufacturing technology. The prosthesis comprises a femoral condyle prosthesis, a tibial plateau prosthesis, a tibial plateau pad prosthesis and a patellar prosthesis. Osseointegration interfaces of the femoral condyle prosthesis, tibial plateau prosthesis and patellar prosthesis is in the structure of the bone induction differentiated metal bone trabecula. The preparation method comprises the steps of determining the gradient structure of stress and strain in knee prosthesis, determining the parameters of the porous structure of trabecular bone, establishing a three-dimensional model of the porous structure, conducting slicing and stratification treatment of the three-dimensional model, and conducting electron beam incremental manufacturing of the knee joint prosthesis metal bone trabecula, cooling to obtain the final finished product. The bone induction differentiated metal bone trabecula knee joint prosthesis and the preparation method thereof for the first time achieve the balance between the stress and bone induction performance for stress regions of different osseointegration interfaces of bone in knee joint, satisfy the differentiation requirement of bone induction, provide a preparation method of mass production, and effectively reduces manufacturing cost.