The invention discloses a highway protective fence rubber protective cover preparation method. The method comprises the following steps that 1, materials are prepared, and the materials comprise high temperature vulcanized rubber, nano zinc oxide, nanosilicon dioxide and a modifier; 2, the vulcanized rubber is placed into a mixing furnace capable of heating, and heating is conducted to 200 DEG C; 3, nano zinc oxide is added and stirred for 10 min; 4, nanosilicon dioxide is added and stirred for 5 min; 5, the modifier is added and stirred for 30 min; 6, the well stirred rubber is poured into a four-roll calender for molding, and a rubber sleeve which is 10-20 mm thick and 300-600 mm long is obtained. The vulcanized rubber is prepared from 78 kg of rubber raw materials, 10 kg of 800-mesh alkali-free glass fiber powder, 6 kg of sulfur, 1 kg of a vulcanization accelerator, 2 kg of phenolic resin powder and 1 kg of hard carbon black. The highway protective fence rubber protective cover is low in cost and resistant to corrosion. The preparation technology is simple in process, low in equipment requirement and high in operability.