The invention discloses endophytic actinomycetes of kiwifruit roots and an application of the endophytic actinomycetes of the kiwifruit roots. The endophytic actinomycetes of the kiwifruit roots are Streptomyces globisporus and named as Streptomyces globisporus SCAU-1, and the collection number of the Streptomyces globisporus SCAU-1 is CCTCC NO: M 2016577. According to the invention, functional endophytic actinomycetes are separated from roots of kiwifruit plants suffering from cankers, thereby promoting plant growth, further being capable of obviously inhibiting the growing abilities of plant pathogenic fungi and pathogenic bacteria, and being capable of being used for preparing a fertilizer-pesticide combined type microbial fertilizer to promote the growth of kiwifruits; three continuous pot experiments show that SCAU-1 fermentation liquor has an obvious growth-promoting effect for kiwifruit seedlings by using a root-irrigation way after being diluted by clear water, so that the root numbers, root lengths, plant heights, leaf numbers and leaf areas during the kiwifruit seedling stage can be obviously improved.