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发明公开
CN106636201A 一种MC1R基因载体及其构建方法 无效 - 驳回

一种MC1R基因载体及其构建方法
摘要：
本发明公开了一种MC1R基因载体及其构建方法，包括MC1R‑neo和MC1R‑GFP；MC1R‑neo核苷酸序列为SEQ ID NO：1；MC1R‑GFP核苷酸序列为SEQ ID NO：2。本发明采用CRISPR/Cas9系统构建MC1R基因敲除载体，只需针对该基因敲除位点设计一个长约20bp左右的sgRNA，连接通用的Cas9基因即可。因此，与传统的ZFNs、TALENs等基因敲除技术比较而言，采用CRISPR/Cas9构建基因敲除载体更为简单快捷，便于进一步推广和应用于后续实验。
摘要（英）：

The invention discloses an MCIR (MelanoCortin 1 Receptor) gene carrier and a construction method thereof. The MCIR gene carrier comprises MC1R-neo and MC1R-GFP (Green Fluorescent Protein), wherein the nucleotide sequence of the MC1R-neo is SEQ ID NO:1, and the nucleotide sequence of the MC1R-GFP is SEQ ID NO:2. A CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 system is adopted to construct an MC1R gene knock-out vector, and only a sgRNA (Ribonucleic Acid) of which the length is about 20bp needs to be designed by aiming at a gene knock-out point to be connected with a universal Cas9 gene. Therefore, compared with traditional gene knock-out technologies including ZFNs (Zinc finger nuclease), TALENs (Transcription Activator-Like Effector Nucleases) and the like, the construction method disclosed by the invention adopting the CRISPR/Cas9 to construct the gene knock-out vector is simpler and more convenient and is more convenient to be further popularized and applied to subsequent experiments.

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