The invention relates to a device for simulating formation of biological sulfate and an experimental method. The device is characterized by comprising a biological sulfate reaction device, an experimental gas generation device, a gas detection and collecting device and a media solution pumping device, wherein the experimental gas generation device which is used for providing test gas for the biological sulfate reaction device communicates with the biological sulfate reaction device through a corrosion-resistant gas duct; the gas detection and collecting device which is used for detecting and collecting generated gas in the biological sulfate reaction device communicates with the biological sulfate reaction device through a corrosion-resistant gas duct; and the media solution pumping device which is used for pumping a media solution of the biological sulfate reaction device communicates with the biological sulfate reaction device through a corrosion-resistant liquid duct. The device can fill technical gap that a special device for simulating formation of the biological sulfate does not exist in the prior art, and the special experimental device is provided for technicians to further know a formation process of the biological sulfate.