The invention applies to the technical field of hormone detection and provides a method, a device and a system for detecting hormones. The method for detecting the hormones is used for detecting the hormones in a to-be-detected substance contacted with a hormone sensor, wherein the hormone sensor comprises a chemiluminescent unit producing a chemiluminescence reaction with the to-be-detected substance and an image collection unit for collecting optical images produced on the chemiluminescent unit, and the optical image corresponding to the to-be-detected substance is obtained if contact between the hormone sensor and the to-be-detected substance is detected; the optical image is compared with a preset optical image, type information of the hormones contained in the to-be-detected substance is determined, so that steps of the hormone detection for the to-be-detected substance are simpler, and the detection efficiency is improved.