The invention discloses a universal organophosphorus pesticide non-enzyme inhibition electrochemistry detection method. The method includes the steps: taking pralidoxime chloride as an organophosphorus electrochemistry probe; detecting oxidation current degree inhibited after the pralidoxime chloride and organophosphorus pesticides are acted, and judging concentration of the organophosphorus pesticides according to the oxidation current degree. According to the method, detection is achieved in buffer solution, a certain amount of pralidoxime chloride is added, oxidation peak current of the pralidoxime chloride is measured, a series of organophosphorus pesticides with different concentrations are added, inhibition ratio is calculated, and a standard curve is drawn and used for measuring organophosphorus pesticides unknown concentrations. Compared with three kinds of existing electrochemistry detection methods of organophosphorus pesticides, the method simultaneously has the advantages of simplicity, convenience, low price, stability, broad spectrum and the like. The method can be used for rapidly detecting organophosphorus pesticides in agricultural products and has a wide application prospect in food safety.