The invention discloses a bidirectional radio-frequency amplifier input-output reverse connection protection circuit and a method thereof. The bidirectional radio-frequency amplifier input-output reverse connection protection circuit comprises a coupler, a radio-frequency wave detector, a comparer, a NOT gate, an AND gate, an NPN triode and a low noise amplifier, and further comprises a radio-frequency input port and a radio-frequency output port; for the radio-frequency input port, the port is connected with a third pin input port of the coupler; a fourth pin output port of the coupler is connected with an input port of the radio-frequency wave detector; a signal output port of the comparer is the input port of the NOT gate; an output port of the NOT gate is the input port of the AND gate; and when the AND gate outputs a low level, the NPN triode is in a cut-off state, and the low noise amplifier is in a power-off state. According to the bidirectional radio-frequency amplifier input-output reverse connection protection circuit and the method thereof disclosed by the invention, when bidirectional radio-frequency input and output are reversely connected, through improving the circuit, the NPN triode does not supply the power to the low noise amplifier; and thus, the damage of a strong signal to the low noise amplifier is avoided, and a device having a low withstanding power behind the low noise amplifier is protected as well.