The invention discloses immune cell cryopreservation liquid and application thereof. The immune cell cryopreservation liquid is prepared from 2 volume percent to 10 volume percent of DMSO (Dimethylsulfoxide), 2 volume percent to 15 volume percent of HSA (Human Serum Albumin), 20 volume percent to 88 volume percent of blood serum, conyza japonica saponin R which is selectively added and the balance of a culture medium. The cryopreservation liquid disclosed by the invention can be used for effectively preserving immune cells and the preservation time is long; after the cells are recovered, the recovery capability is high and the recycling rate of the cells is high.