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发明授权
CN106676079B 7α-羟基类固醇脱氢酶基因S1-a-2 有权

7α-羟基类固醇脱氢酶基因S1-a-2
摘要：
本发明涉及羟基类固醇脱氢酶，具体涉及一种7α‑羟基类固醇脱氢酶基因S1‑a‑2。该基因的核苷酸序列如SEQ ID NO.2所示，编码一种7α‑羟基类固醇脱氢酶，其氨基酸序列如SEQ ID NO.1所示，能够催化鹅去氧胆酸(CDCA)、牛磺鹅去氧胆酸(TCDCA)生成7‑酮石胆酸(7K‑LCA)、牛磺7‑酮石胆酸(T7K‑LCA)，对CDCA或TCDCA的催化活性约为撒丁岛梭菌7α‑HSDH的2倍，具有很大的工业应用价值。
摘要（英）：

The invention relates to hydroxysteroid dehydrogenase, in particular to a novel 7alpha-hydroxysteroid dehydrogenase gene S1-a-2. A nucleotide sequence of the gene is shown as SEQ ID NO.2. A novel 7alpha-hydroxysteroid dehydrogenase with an amino acid sequence shown as SEQ ID NO.1 is coded and is capable of catalyzing CDCA (chenodeoxycholic acid) and TCDCA (taurochenodeoxycholic acid) to generate 7K-LCA (7-ketone lithocholic acid) and T7K-LCA (tauro-7-keto lithocholic acid), catalytic activity of the novel 7alpha-hydroxysteroid dehydrogenase for the CDCA or TCDCA is about twice of that of Clostridium sardiniense 7alpha-HSDH for the same, and accordingly the novel 7alpha-hydroxysteroid dehydrogenase is high in industrial application value.

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