The invention provides a method for normalization of transformer winding vibration accelerations at different temperatures. Winding vibration signals of a transformer at different oil temperatures are obtained; a relation curve of the temperature of the transformer oil and winding vibration acceleration fundamental frequency amplitude is established, and a slope of curve is utilized to normalize the transformer winding vibration accelerations at the different temperatures. Through the normalization of the transformer winding vibration accelerations at the different temperatures, a power transformer fault diagnosis technology is improved, and manpower and material resources are improved.