The invention discloses a ternary weeding composition containing glyphosate and application thereof. Effective active components comprise a first effective active component, namely the glyphosate, a second effective active component, namely glufosinate-ammonium, and a third effective active component, namely fluoroglycofen-ethyl, wherein the mass ratio of the glyphosate to the glufosinate-ammonium to the fluoroglycofen-ethyl is (1-90) to (1-90) to (1-90). The composition can be prepared into agriculturally acceptable wettable powder, water dispersible granules, a suspending agent, a dispersible oil suspending agent, suspension emulsion, missible oil and an aqueous solution. The ternary weeding composition has reasonable components and has a very good non-tillage weeding effect; the activity and the weeding effect are not simple superposition of the activity of all the components; compared with an existing single preparation or a binary compound preparation, the ternary weeding composition has a remarkable weeding effect and also has a remarkable synergistic effect; the pesticide dosage is reduced, a weeding spectrum is widened and the prevention and control cost is low.