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发明公开
CN106693443A 一种维生素B12适配体亲和柱及其制备方法和用途 无效 - 驳回

一种维生素B12适配体亲和柱及其制备方法和用途
摘要：
本发明涉及一种维生素B12适配体亲和柱及其制备方法和用途。该亲和纯化柱利用化学修饰的固相载体，然后用维生素B12的核酸适配体与载体进行共价偶联。然后装填亲和柱。该适配体亲和柱主要用于对于食品、奶粉、牛奶、饮料以及其他多种样本中的维生素B12的纯化，以便于后期对样本中维生素B12的高效液相色谱（HPLC）检测和质谱检测。
摘要（英）：

The invention relates to a vitamin B12 aptamer affinity column, and a preparation method and application thereof. The preparation method comprises the following steps: by using a chemically-modified solid-phase carrier, carrying out covalent coupling on a vitamin B12 aptamer and the carrier; and filling the affinity column. The aptamer affinity column is mainly used for purifying vitamin B12 in food, milk powder, milk, beverages and many other samples, thereby facilitating the high performance liquid chromatography (HPLC) detection and mass spectrometric detection on vitamin B12 in the sample in the later period.

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