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发明公开
CN106701682A 由脐血分离造血干细胞并扩增CD34阳性细胞的方法 无效 - 撤回

由脐血分离造血干细胞并扩增CD34阳性细胞的方法
摘要：
本发明涉及由脐血分离造血干细胞并扩增CD34阳性细胞的方法。具体包括如下步骤：脐血造血干细胞的分离；纯化；Ficoll分离液分离；流式细胞仪进行CD34阳性细胞计数、稀释、接种、培养；换液培养；分板培养；评估细胞扩增效果。还涉及包括该方法制得的CD34阳性细胞的细胞制品，以及它们的用途。本发明方法具有如说明书所述优异技术效果。
摘要（英）：

The invention relates to a method for separating hematopoietic stem cells from umbilical cord blood and amplifying CD34 positive cells. The method comprises the following specific steps: separating hematopoietic stem cells from umbilical cord blood; purifying; separating by using a Ficoll separating solution; counting the CD34 positive cells by using a flow cytometer, diluting, inoculating and culturing; changing the solution and culturing; transferring the cells to new plates and culturing; evaluating the cell amplifying effect. The invention further relates to cell products containing the CD34 positive cells prepared by the method and application of the cell products. The method has excellent technical effects as described in the specification.

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