The invention provides a method for determining content of six ginsenoside ingredients of folium ginseng. Ginsenoside is the main active ingredient of the folium ginseng and an important criterion for measuring the quality of the folium ginseng, but the content is greatly different from that of ginseng. According to the method, ultra-high performance liquid chromatography is used for determining the six ginsenoside ingredients including ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rb2, 20(S)-ginsenoside F1 and ginsenoside Rd in the folium ginseng simultaneously, the method is high in sensitivity and accurate, and can be used for quality control of the folium ginseng.