The present invention discloses a microscope shooting assisting device. The microscope shooting assisting device comprises a base; two second upright pillars are arranged on the base; the second upright pillars are arranged in parallel; the top end of each second upright pillar is connected with a first upright pillar by a height adjusting mechanism; a baffle is arranged at the tops of the first upright pillars; the baffle is provided with a bracket; the baffle is arranged at the bottom of the bracket; the baffle and the bracket are arranged in a manner that the baffle is perpendicular to the bracket; and the baffle is connected with an angle adjusting mechanism. The microscope shooting assisting device is made of metal or hard plastic, low in cost and easy to arrange and occupies less space; the microscope shooting assisting device can be compatible with shooting devices of various kinds of brands and models, such as microscopes, mobile phones, cameras and small cameras; and the device is simple to operate. With the device adopted, height, angles and shooting distances can be adjusted; a shooting effect can be stable, an obtained image is clear and complete; and length quantification can be performed through using the diameter distance of a field of view in an imaged picture, and therefore, shooting time can be decreased, shooting difficulty can be reduced, and shooting efficiency can be improved.