The present invention relates to: a method for cutting a separation membrane for a battery, in which the separation membrane is cut by laser radiation on the separation membrane, wherein the pulse repetition rate of the laser is 10 to 500 kHz; a separation membrane manufactured by the method; and a battery comprising the separation membrane. The present invention, in contrast with physical cutting, has the advantage of being capable of cutting a separation membrane for a battery so as to have a uniform cut surface, which was impossible by conventional physical cutting methods.