The invention discloses a preparation method of multi-bitter-modified bitter gourd whole fruit glucose-reducing powder. The bitterness of a bitter gourd is reduced with multiple bitter modification methods, wherein multiple bitter modifications comprise bitter inhibition, adsorption and embedding. Besides, the invention discloses the multi-bitter-modified bitter gourd whole fruit glucose-reducing powder prepared with the method. In combination of the multiple bitter modification methods, the bitterness can be substantially reduced without damaging the active substances of the bitter gourd. Besides, adsorption and embedding modes are adopted in the preparation process, which are favorable for conserving active ingredients, and the nutrition and activity of a product are improved. The multi-bitter-modified bitter gourd whole fruit glucose-reducing powder has bitter gourd whole fruits as a raw material, and auxiliary materials added in the bitter modification process are favorable for glucose control; the bitterness is reduced by 85% or above while the active substances of the bitter gourd are kept to the greatest extent, no bad flavor is introduced, and the glucose-reducing powder as a glucose-reducing function factor is added to a special food for diabetics.