Provided is an anastomotic/closed stoma reinforcement and repair assembly, comprising a reinforcement and repair part (11), a protective part (12) and a connection wire (13), wherein the connection wire (13) detachably connects both ends of the reinforcement and repair part (11), and the protective part (12) is inserted into the space enclosed by both ends of the reinforcement and repair part (11). At the same time, further provided is a method for preparing and using the anastomotic/closed stoma reinforcement and repair assembly. The anastomotic/closed stoma reinforcement-repair assembly can not only provide, for a tissue to be treated, a sufficient strength to prevent a suture from tearing, but also has a lower pathogenicity and a high biocompatibility with little or no immunogenicity, and maintains numerous beneficial components for promoting tissue repair.