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发明公开
CN106750332A 星形聚合物接枝多肽和聚乙烯亚胺的基因载体及制备方法 无效 - 撤回

星形聚合物接枝多肽和聚乙烯亚胺的基因载体及制备方法
摘要：
本发明公开了星形聚合物接枝多肽和聚乙烯亚胺的基因载体及制备方法，方法为：将山梨醇、LA、MMD和辛酸亚锡放入聚合管中，密封；抽真空，通氮气；反应，得到P(LA‑co‑MMD)粗品；粗品用三氯甲烷溶解，正己烷沉淀；得到P(LA‑co‑MMD)；制备端基为羧基的P(LA‑co‑MMD)；制备P(LA‑co‑MMD)‑g‑PEI；制备P(LA‑co‑MMD)‑g‑PEI‑g‑PEG‑CREDVW；制备星形聚合物接枝多肽和聚乙烯亚胺的基因载体，本发明的基因载体低的细胞毒性，有效地负载质粒，具有对内皮细胞的靶向性，选择性的促进内皮细胞的增殖和迁移，从而达到快速内皮化。
摘要（英）：

The invention discloses a gene vector of star polymer grafted polypeptide and polyethyleneimine as well as a preparation method thereof. The preparation method comprises the following steps: putting sorbitol, LA (lactide), MMD and stannous octoate into a polymerization tube for sealing; performing vacuumization and pumping in nitrogen; reacting to obtain crude P(LA-co-MMD); dissolving the crude P(LA-co-MMD) by trichloromethane, and precipitating the crude P(LA-co-MMD) by n-hexane; obtaining P(LA-co-MMD); preparing P(LA-co-MMD) with carboxyl as a terminal group; preparing P(LA-co-MMD)-g-PEI; preparing P(LA-co-MMD)-g-PEI-g-PEG-CREDVW; and preparing the gene vector of the star polymer grafted polypeptide and the polyethyleneimine. The gene vector disclosed by the invention has the advantages of low cytotoxicity, capability of loading plasmid effectively and selectively promoting proliferation and migration of endothelial cells so as to realize rapid endothelialization, and has targeting performance for the endothelial cells.

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