The application provides a composition of leather, the leather and a preparation method of the leather. The composition comprises a bottom-layer composition and a surface-layer composition, wherein the bottom-layer composition is prepared from a first plasticizer; the surface-layer composition is prepared from a second plasticizer; metered in parts by weight, the first plasticizer is 6 to 30 parts more than the second plasticizer; further, the parts by weight of the second plasticizer are less than 85 parts. According to the leather made from the leather composition, the scratch resistance performance of the leather is improved; further, a creasing-folding phenomenon is enabled to difficultly appear on the surface of the leather.