The invention relates to a plant callus culture utensil which comprises a petri dish and an upper cover, wherein the petri dish is in seamless gap connection with the upper cover; a detachable recyclable connecting strip for fixation is arranged between the petri dish and the upper cover; convex points in mutual symmetry are arranged between the petri dish and the upper cover to achieve buckle type fixed connection of the petri dish and the upper cover; a columnar ventilation cavity layer is arranged right in the middle of the upper surface of the upper cover; the columnar ventilation cavity layer is provided with a cover part with small vent holes and a bottom which is integrated with the upper cover of the petri dish in an injection molding manner and is provided with a ventilation membrane; and the cover part with the small vent holes can be opened to replace degreased cotton or the ventilation membrane inside the cavity layer. The plant callus culture utensil is capable of effectively solving the defect that the cover plate and the bottom of a conventional petri dish are not stable, and is simple to process, environmentally friendly and effective, convenient to operate, beneficial to sunshine radiation, capable of effectively preventing bacterium invasion to damage plant tissue and ensuring circulation of air and moisture, and beneficial to culture of plant callus tissue.