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发明公开
CN106754388A 一种拟微绿球藻及其驯化方法和应用 无效 - 驳回

一种拟微绿球藻及其驯化方法和应用
摘要：
本发明公开一种拟微绿球藻(Nannochloropsissp)ENN14‑1(保藏编号CGMCC No.13384)及其驯化方法和应用，涉及微生物工程技术领域，以使拟微绿球藻在淡水区域规模化经济养殖。所述拟微绿球藻的驯化方法包括：逐级降低驯化培养基的盐度对拟微绿球藻藻种进行多级驯化，得到多级驯化的拟微绿球藻藻种；其中，一级驯化所使用的驯化培养基盐度小于海水培养基盐度，且大于等于海水培养基盐度的四分之一，最后一级驯化所使用的驯化培养基盐度等于0.5％；在淡水培养基中对多级驯化的拟微绿球藻藻种进行培养，获得拟微绿球藻。本发明提供的拟微绿球藻及其驯化方法和应用，用于拟微绿球藻的淡水规模化养殖。
摘要（英）：

The invention discloses a nannochloropsissp ENN14-1 (CGMCC No.13384) as well as a domestication method and application thereof, and relates to the technical field of microorganism engineering, in order to realize the large-scale economic cultivation for the nannochloropsissp in a fresh water area. The domestication method for the nannochloropsissp comprises the following steps: gradually reducing the salinity of a domestication culture medium, and performing multi-level domestication on nannochloropsissp, thereby acquiring multi-level domesticated nannochloropsissp alga species, wherein the salinity of the domestication culture medium used for first-level domestication is lower than the salinity of a seawater culture medium and is more than or equal to one quarter of the salinity of the seawater culture medium, and the salinity of the domestication culture medium used for last level domestication is equal to 0.5%; and culturing the multi-level domesticated nannochloropsissp alga species in a fresh water culture medium, thereby acquiring the nannochloropsissp. The nannochloropsissp as well as the domestication method and application thereof provided by the invention can be applied to fresh water large-scale culture of the nannochloropsissp.

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