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发明公开
CN106755494A 蜂房蜜蜂球菌荧光定量PCR检测试剂盒及其检测方法 审中-实审

蜂房蜜蜂球菌荧光定量PCR检测试剂盒及其检测方法
摘要：
本发明涉及蜂房蜜蜂球菌荧光定量PCR检测试剂盒及其检测方法，专用于蜂房蜜蜂球菌的检测。该试剂盒包括：（1）阳性标准品；（2）阴性对照；（3）PCR反应液；（4）ExTaq酶；（5）无菌水。本发明具有以下优点：（1）良好的稳定性和特异性：对蜂房蜜蜂球菌的检测具有高度特异性，与其它蜜蜂病原菌无交叉反应，且重复性好；（2）灵敏度高：灵敏度能达到50拷贝/μL；（3）操作简便、快速：整个反应可在2小时内完成。本发明试剂盒可用于蜜蜂欧洲幼虫腐臭病的诊断及其蜂房蜜蜂球菌的检测，对该病的早期预防和及时治疗具有重要意义。
摘要（英）：

The invention relates to a fluorogenic quantitative PCR detection kit for melissococcus pluton and a detection method thereof. The kit is especially used for detecting melissococcus pluton. The kit comprises (1) a positive standard substance, (2) a negative control, (3) a PCR reaction solution, (4) ExTaq enzyme and (5) aquae sterilisata. The invention has the advantages of (1) high stability and specificity: high specificity in detection for melissococcus pluton, no cross reaction with other bee pathogenic bacteria and excellent repeatability, (2) high sensitivity capable of reaching up to 50 copy/uL and (3) simple, convenient and quick operation: capability of completing the whole reaction within 2 hours. The kit provided by the invention can be used for diagnosing bee European foul brood and melissococcus pluton and has significance in early prevention and timely treatment of the diseases.

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