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发明公开
CN106755565A 一种基于荧光PCR法联合检测登革热病毒及基孔肯亚病毒的试剂盒 无效 - 驳回

一种基于荧光PCR法联合检测登革热病毒及基孔肯亚病毒的试剂盒
摘要：
本发明涉及荧光PCR检测试剂盒领域，特别是涉及一种基于荧光PCR法联合检测登革热病毒及基孔肯亚病毒的试剂盒。本发明提供一种联合检测登革热病毒及基孔肯亚病毒的试剂盒，所述试剂盒包括针对登革热病毒的特异性引物、登革热病毒探针、针对基孔肯亚病毒的特异性引物以及基孔肯亚病毒探针，所述针对登革热病毒的特异性引物包括正向引物和反向引物，所述针对基孔肯亚病毒的特异性引物包括正向引物和反向引物。本发明所提供的试剂盒具体涉及两对特异性PCR引物和探针，分别特异性扩增登革热病毒和基孔肯亚病毒，两对特异性PCR引物能在同一PCR反应体系中同时进行扩增，实现了多重PCR检测，不仅操作简便，还大大缩短了检测时间。
摘要（英）：

The invention relates to the field of PCR detection kits, in particular to a kit for joint detection on a dengue fever virus and a chikungunya virus on the basis of a fluorescent PCR method. The kit for joint detection on the dengue fever virus and the chikungunya virus comprises a specific primer for the dengue fever virus, a dengue fever virus probe, a specific primer for the chikungunya virus and a chikungunya virus probe, wherein the specific primer for the dengue fever virus comprises a forward primer and a reverse primer; the specific primer for the chikungunya virus comprises a forward primer and a reverse primer. The kit provided by the invention specifically relates to two pairs of specific PCR primers and probers for respectively specifically amplifying the dengue fever virus and the chikungunya virus, the two pairs of the specific PCR primers can amplify the viruses simultaneously in a same PCR reaction system, multi-PCR detection is achieved, and not only is operation simple and convenient, but also the detection time is greatly shortened.

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