The invention provides a microscopic flowability evaluation method of high-viscosity low-fluidity tight oil under different temperature and pressure conditions. The detection of the T2 spectrum of the sample under different conditions is combined with analysis evaluation to obtain the microscopic flowability condition of the high-viscosity low-fluidity tight oil under different temperature and pressure conditions. The evaluation method can be used for recognizing the microscopic flowability behavior of the high-viscosity low-fluidity tight oil under different temperature and pressure conditions in a comprehensive, systematic and reliable way.