The invention relates to a kit for rapidly diagnosing lipoprotein-associated phospholipase A2 and a use method of the kit. The kit comprises a standard substance, a quality control substance, a liposome complex, a magnetic separation reagent, an assay buffer solution and a cleaning solution, wherein the inner part of the liposome complex is coated with N-hydroxysuccinimide ester of tris (bipyridine) ruthenium; an antibody for resisting the lipoprotein-associated phospholipase A2 is connected to the surface of the liposome complex by interaction of streptavidin and biotin; the magnetic separation reagent takes magnetic particulates containing amino terminals as a carrier and is connected with the antibody for resisting the lipoprotein-associated phospholipase A2 through the interaction of the streptavidin and the biotin. Compared with an enzyme linked immunosorbent assay kit produced in a foreign Diadexus company, the kit prepared by the invention has the advantages that sensitivity and precision for analyzing the lipoprotein-associated phospholipase A2 are greatly improved; in addition, the kit disclosed by the invention is simple and convenient to operate, quick and easier to popularize.