The invention relates to a preparation method of yeast capable of coupling antibodies. Sodium periodate, a bishydrazide compound, a dialdehyde compound, and an acidic buffer are used in order to prepare yeast capable of coupling antibodies. The yeast with biological coupling modification can be combined with antibodies, and can be applied to the biotechnology fields of flow-type microsphere immunoassay, ELISA analysis, electrochemiluminescence/chemiluminescence immune assay, affinity chromatography, immunoprecipitation and the like. The yeast capable of coupling antibodies has substantial advantages of simple, high efficiency and stabilization.